Sentence examples for min for determination from inspiring English sources

Plasma was drawn every 5 min for determination of plasma glucose, and glucose infusion was adjusted appropriately to maintain the fasting glucose level.

After a 10-h overnight fast, all subjects underwent a 75-g OGTT, and venous blood samples were obtained at 0, 30, 60, 90, and 120 min for determination of plasma glucose, insulin, proinsulin, and C-peptide.

Various concentrations of PGF2α and of its synthetic analogue, cloprostenol were applied during 20 min. Aliquots of the perifused media were collected every 10 min for determination of corticosterone concentrations (Fig. 5C).

After a 10-h overnight fast, all subjects underwent a 75-g OGTT and venous blood samples were obtained at 0, 30, 60, 90, and 120 min for determination of plasma glucose, insulin, and C-peptide.

From this point, samples were taken regularly every 10 20 min for determination of nitrate concentrations.

Venous blood samples were obtained at 0, 30, 60, 90 and 120 min for determination of plasma glucose, insulin and C-peptide concentrations and 0, 30 and 120 min for determination of GLP-1 concentrations.

Serum was prepared by centrifugation within 1 h at 2,500 g for 10 min for determinations of CRP, tumor necrosis factor-α (TNF-α), IL-6, IL-8, IL-10, IL-18, and adiponectin.

Cycle conditions were 45 s at 96°C for initial denaturing followed by 25 or 30 cycles at 96°C for 30 s, 58 72°C (primer specific annealing temperature) for 30 s and 72°C for 1 min and a final extension step at 72°C for 5 min. For determination of alternative polyadenylation sites the 3'RACE method was used.

During the last 40 min of both hyperinsulinaemic periods, blood samples were drawn at 10 min intervals for determination of isotope enrichments, glucoregulatory hormones and NEFA.

All mixtures were kept at 24°C for up to 120 min. For determination of the remaining enzyme activity, samples were taken after 1, 30, 60 and 120 min and measured using the veratryl alcohol assay mentioned above.

The temperature activity profile of free and entrapped laccase was determined at different temperatures ranging from 25-75°C 25-75°Cmin. for determination of half-lives the enzymes were incubated at 60°C for varying time periods before carrying out standard laccase assay.