Sentence examples for min for denaturation from inspiring English sources

Genomic DNA was used as a template for PCR amplification with cycling conditions of 94°C for 5 min as hot start; 35 cycles of 94°C at 1 min for denaturation; 55 60°C annealing for 1 min; 72°C extension for 2 min; and a 7 min final extension at 72°C.

The sequencing reaction consisted of an initial 5 min for denaturation at 96°C, 25 cycles of 96°C for 15 sec, 50°C for 10 sec, 60°C for 4 min and elongation time of 72°C for 15 min using the BigDye 3.1 Terminator Cycle Sequencing Kit (Applied Biosystems Inc).

RT-PCR amplification was performed using an ABI 7300 (Applied Biosystems, Foster City, CA, USA) with the following thermal cycling conditions: 2 min at 50°C, 10 min at 95°C, followed by 40 cycles of 95°C for 15 s and 60°C for 1 min for denaturation, annealing, and elongation.

Metaphase spreads were washed in PBS for 15 min and fixed in 4% paraformaldehyde/PBS for 2 min. After dehydration, a Cy3-telomere PNA probe (10 nM) was added, and the samples were incubated at 85°C for 5 min for denaturation, and then in hybridization solution (20 mM Tris-HCl (pH 7.2), 70% formamide, and 1% BSA) overnight at room temperature in the dark.

The PCR tube was incubated at 95°C for 5 min for denaturation using an ABI GeneAmp PCR System 9700 (Applied Biosystems, Life Technologies Japan, Ltd).

Each sample pellet was resuspended in 12.5 µL of template suppression reagent and heated at 95°C for 3 min for denaturation.

The PCR conditions were set to 1 min each for denaturation at 95 °C, annealing at 55 °C, 2 min for an extension at 72 °C and finally finishing with a 10 min step at 72 °C.

The reaction was cycled according to the following profile: 1) precycling: 20°C for 5 min and 95°C for 5 min; and, 2) 99 cycles of 95°C for 15 s and 65°C for 12 min allowing for denaturation, primer annealing, extension and ligation.

Volumes of 50 μl were set up under the following PCR-conditions: 94°C for 2 min of initial denaturation, 30 cycles of 94°C for 1 min of denaturation, 54°C for 1 min of annealing and 72°C for 1 min of amplification; followed by a final extension of 72°C for 10 min. PCR products were run in a 1% agarose gel (Lonza, Rockland ME, USA) and stained with ethidium bromide (0.07%, Sigma).

The PCR reaction was for 2 min of 94°C preheating, followed by a 30 cycle amplification program (1 min at 94°C for denaturation, 1 min at 58°C for annealing, and 1 min at 72°C for extension) and a final extension at 72°C for 5 min.

The reactions were performed according to the recommendations of the manufacturer: 95 °C for 5 min for initial denaturation, followed by 45 thermal cycles of 10 s at 95 °C, 10 s at 60 °C and 20 s at 72 °C.