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Each session was divided into 20 min for completing modules demonstrating pathology on SonoSim® machines and 20 min for facilitator-led hands-on practice with a SonoSite Edge® machine and volunteer models.

Thereafter, 0.5 ml of the reaction mixture containing nitrite was pipetted and mixed with 1 ml of sulfanilic acid reagent (0.33% sulfanilic acid in 20% glacial acetic acid) and allowed to stand for 5 min for completing diazotization.

After incubation, 0.5 ml of the reaction mixture containing nitrite was pipetted and mixed with 1 ml of sulphanilic acid reagent (0.33% in 20% glacial acetic acid) and allowed to stand for 5 min for completing diazotization.

The samples were allowed to stand for 15 min for complete color development.

The mixture was stirred for another 30 min for complete reaction then the solution was neutralized.

This method needs hardly 3 to 5 min for complete synthesis without using any complication.

After incubating for another 3 h at 37 °C, the medium was withdrawn and replaced with 200 μL DMSO and allowed to stand about 15 min for complete reaction.

The maximum convective stirring settings correspond to only 17 min for complete mixing of the mesocosm water (Fig. 8f) with still a rather uniform temperature profile (Fig. 8e).

With ZnO = 0.20 g/40 mL and [CV] = 2.00 × 10−5 M, it needs about 360 min for complete mineralization of CV molecules in the suspension.

This was evident from its slow and gradual color change when reduced, taking approximately 7 min for complete reduction to occur.

The optimum values of pH, adsorbent dose, initial dye concentration and time are found to be 10, 2.1846 g/L, 50 mg/L and 63 min for complete removal of MB dye respectively.