Sentence examples for min for antigen from inspiring English sources

Slides were then placed in 10 mM sodium citrate buffer at 95°C for 20 min for antigen retrieval.

For IHC staining, the tissue section was deparaffinized and rehydrated, and boiled in 10 mM citrate buffer for 30 min for antigen retrieval.

Sections stained for collagen I and II were incubated with 0.1% w/v hyaluronidase (Sigma-Aldrich) at 37 °C for 30 min for antigen retrieval.

Briefly, after treatment with pepsin for 10 min for antigen retrieval, sections were incubated with anti-TNC antibody 4F10TT and subsequently with peroxidase-conjugated anti-mouse IgG Fab' (Medical and Biological Lab Co. Ltd., Nagoya, Japan).

The slides were incubated with 10 mM citrate buffer (pH 6.0) and microwaved for 20 min for antigen retrieval.

After paraffin removal, sections were heated in a water bath in sodium citrate buffer 0.1 M, pH 6.0 for 40 min for antigen retrieval.

For IHC, FFPE sections were deparaffinized and rehydrated, treated for endogenous peroxidases (3%H2O22, 10 min), processed for antigen retrieval (Proteinase K, 20 μg/ml, 7 min, Fermentas or heat treat in TE for 1 hr at 70°C), blocked for 30 min with 10% horse serum/0.2% Triton X-100, and incubated with primary antibody (overnight 4°C, see Table S1).

Endogenous peroxidase activity was quenched with 3% hydrogen peroxide at room temperature for 15 min. For antigen retrieval, Borg Decloaker (Biocare Medical Inc., Walnut Creek, CA, USA) was applied for 2 min. Slides were then blocked with normal goat serum, and anti-rat CD3 antibody or anti-rat CD45RA antibody (both from AbD Serotec, Oxford, UK) was applied at 1 100 dilution.

Endogenous peroxidase activity was then blocked with methanol containing 3%H2O22 for 20 min. For antigen retrieval, sections were treated with citrate buffer saline (pH 6.0) for 15 min at 95°C in a microwave oven.

Finally, sections were washed with running water with blocking by 3% hydrogen peroxide for 10 min. For antigen retrieval, tissue sections were boiled in citrate buffer solution (pH 6.0) for 3 min. Sections were then washed in phosphate buffer solution (PBS) three times, followed by probing with anti-EpEX and anti-EpICD (1 100 dilution) at 37 °C for 1 h, respectively.

Further, incubation was done in 0.3% hydrogen peroxide in methanol solution for 45 min. The slides were washed with citrate buffer and kept in a water bath at 90 95°C for 45 min. for antigen retrieval.