Sentence examples for min for activation from inspiring English sources

Unless otherwise stated, incubation times with ET-1 were dependent upon the time at which individual signaling components are substantially activated: 30 s for RhoA.GTP loading [25], 5 min for activation of ERK1/2 [26] or p38-MAPKs [27], 15 min for activation of JNKs [28] and 1 h for expression of immediate early genes [10].

The reaction mixture was heated at 95°C for 9 min (for activation of AmpliTaq Gold), followed by 70 cycles of amplification using Mastercycler (Eppendorf AG, Hamburg, Germany).

The conditions for PCR were as follows: 95°C for 10 min for activation, followed by 40 cycles of denaturation at 95°C for 15 s each, and finally, primer extension at 60°C for 1 min.

The PCR ran for 1 cycle at 95°C for 10 min for activation of the DNA polymerase, followed by 2 cycles of 15 sec at 94°C and 2 min at 49°C, and 30 cycles of 15 sec at 94°C, 20 sec at 62°C and 1 min 40 sec at 74°C.

The cycling parameters used were as follows: one cycle at 50°C for 3 min for activation of UNG, one cycle a 95°C for 5 min for DNA polymerase activation, and 45 cycles of 95°C for 15 sec (denaturation) and 60°C for 1 min (annealing and extension).

Cycling conditions were the same for seven transcripts (18S, CYP19, PPARγ, AhR, CYP1B1, 17β -HSD, HCG): 95.0°C for 5 min for activation of the enzyme, 95.0°C for 30 sec for denaturation and 60.0°C for 1 min for annealing/extension for 40 cycles, followed by a dissociation step.

TaqMan analysis were realized by using the FastStart DNA Master Hybridization Probes kit (Roche Diagnostics), with the following amplification program: 10 min – 95°C for activation of the enzyme, 40×[95°C – 10 sec; 60°C – 10 sec; 72°C – 10 sec].

Amplification was done by using the following program: 10 min – 95°C for activation of the enzyme, 40×[95°C – 10 sec; X°C – 4 sec (see Table S1 in supplementary data); 72°C – 5 sec] for amplification, and [95°C – 0 sec; 65°C – 15 sec, increased to 95°C by 0.1°C/sec] for melting curves analysis.

In the case of DOPA-functionalized surface (SD), the aminated microcantilever was treated with glutaraldehyde (GA, 10% in phosphate-buffered saline (PBS)) for 30 min for surface activation and then immersed into the DOPA solution (65 mM in PBS at pH 7.4) for 10 h [16].

A mixture of 5 6 -carboxyfluorescein (19 mg, 5 6 -carboxyfluoresceiniazole-19yl)-1,1,3,3-tetramgthyluronium hexafluorophosphate (HBTU) (18 mg, 47.50μmol), and N,N-diisopropylethylamine (DIPEA) (2- 1H-benzotriazole-1-yl -1,1,3,3-tetramethyluronium incubated for 10 min for carboxyl activation.

PCR reactions were performed as described previously [19] with an initial incubation step at 95°C during 5 min for the activation of the HotStar Taq Plus DNA Polymerase.