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That night, when Min failed to fall asleep, she opened the man's e-mail from the night before.
In one rat study, nitroglycerin (NTG) in doses of 2 and 50 μg/kg per min failed to change CGRP levels in the jugular vein indicating that NTG exerts its migraine- or cluster headache-inducing effect without involving this neuropeptide [38].
A single stimulation for 90 min failed to alter PGC-1α expression significantly (Fig. 1A).
Second, SSt to either Schaffer or commissural pathway alone (i.e. Δt = 60 min) failed to affect synaptic efficacy in either pathway (see Fig. S1A).
Further analyses with repeated measures using smaller temporal increments (5 min) failed to show any transient differences in Ca2+ concentration between hippocampal neurons exposed to the GSM 2 W/kg or control condition (F(2.11, 21.14) = 0.87, p = 0.44).
A dim light pulse at early subjective night (CT15) (10 or 20lx, 5 min) induced phase delay of locomotor activity rhythms in Adcyap1−/− mice, similar in magnitude to that in Adcyap1+/+ mice, but a brighter pulse (100lx, 5 min) failed to produce any further shifting in the mutants (Fig. 1A and Fig. S1A).
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Finally, two transcripts with short half lives but lacking obvious GREs, Myc (t½ = 30 min) and Plk2 (t½ = 34 min), also failed to show enrichment in the CUGBP1 immunoprecipitation (Figure 4A).
Increased antiseptic concentrations of 1% PHMG-P and 0.2% CHX after 5 min exposure failed to produce a bactericidal effect.
Another radiofrequency machine, operated at 42 °C for 30 min, also failed to have cause significant apoptosis in cells (unpublished data).
Patients were considered for lumbar CSF drainage if they suffered persistent intracranial hypertension (>25 mmHg) for a period of more than 15 min and failed to respond to high intensity treatment.
In contrast, following perfusion with 30 μ m NS1643 for 20 min, PES failed to induce VT with a persistence of normal regular rhythm in 7 of 13 (54%) hypokalaemic hearts (Fig. 4a).
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