Sentence examples for min extension time from inspiring English sources

A final 10 min extension time at 72°C was also performed.

PCRs were done with 22 cycles and 3 min extension time, and the products were analysed on a 1% (w/v) agarose gels.

PCR was conducted for 25 cycles with primer P1 and P2 under the conditions described above with 3 × Phusion enzyme and 7 min extension time.

Reaction conditions were: 95°C for 2 min, followed by 30 cycles of 95°C for 30 s, 50°C for 40 s and 72°C for 2 min, followed by 10 min extension time at 72°C.

PCR was performed as follows: 1 cycle of 94°C for 2 min, then 35 cycles of 94°C for 30 sec, 40°C for 30 sec, 70°C for 1 min 30 sec, followed by a 3 min extension time at 70°C (Biometra T3 thermocycler, Göttingen, Germany).

The PCR program was as follows: 95 °C for 5 min and then 35 cycles at 95 °C for 60 s, 52 °C for 45 s and 72 °C for 90 s, followed by a 10-min extension time at 72 °C.

Standard cycling conditions were 5 min initial denaturation at 95° followed by 30 cycles of 0.5 min at 94°, 0.5 min annealing at the appropriate Tm, and a 2-min extension time (Table 2).

Thirty cycles were performed, including denaturation at 95°C for 1 min, annealing at 59°C for 30 s, and extension at 72°C for 2 min, followed by a 10-min extension time at 72°C.

A touchdown PCR cycling program was used where the annealing temperature was gradually lowered from 70 to 56 °C in 2 °C increments with a 3-min extension time for each cycle.

The cycling conditions were 94°C for 5 min following 35 cycles of 94°C for 50 s, 50°C to 65°C for 50 s (primer-specific annealing temperatures, see Table  1), 72°C for 1 min, following a 10-min final extension time at 72°C.

After the addition of primers an additional 35 cycles of 94°C for 15 s, 62°C for 20 s, 68°C for 3.5 min were performed, followed by a final extension of 3 min. Extension times were varied according to PCR product lengths.