Sentence examples for min each wash from inspiring English sources

The slides were blocked by incubation in 100 mM ethanolamine in 50 mM sodium borate, pH 8.0 for 1 h, then washed three times in PBS, pH 7.4, with 0.05% Tween-20 (PBS-T) for 5 min each wash and once with Tris-buffered saline supplemented with Ca2+ and Mg2+ ions (TBS; 20 mM Tris-HCl, 100 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, pH 7.2).

The strips were washed five times (5 min each wash) before antigen detection proceeded as outlined above for immuno-blotting.

Worms were washed 3 times in cacodylate buffer for 10 min each wash, followed by a secondary fixation in 1% osmium tetroxide for 2 hours at room temperature.

SDH activity was detected as follows: the gels were washed 3 times in 0.1 M Tris buffer (pH 8.5) for 10 min, each wash, at 4°C.

The membrane was washed in 0.2 M wash buffer (0.2 M Na2HPO4 pH 7.2, 1 mM EDTA, and 2% SDS), twice at room temperature and once at 40°C (5 min each wash), and exposed to film.

After hybridization, the gel was washed three times with 4× SSC and once with 4× SSC and 0.1% SDS at 50°C (30 min each wash), and exposed to PhosphoImager or film.

Once fixed and bleached as described in the previous section, the animals were rehydrated through a series of MeOH/PBSTx-0.3MeOH/PBSTx-0.3%0, 250 MeOH in 0.3% Triton-X/1X PBS in MilliQ water) for 10 min each, washed twice with PBST-0.3% for 10 min, and then blocked in 1% BSA in PBST-0.3% for 25h.

Cells were blocked in 1% BSA-PBS followed by incubation with goat anti-GFP (2.8 µg/mL) in PBS-BSA for 30 min each, washed (3×) with PBS and finally incubated with Streptavidin APC-Cy7 at the same concentration for 30 min at RT.

The cells were fixed and permeabilised with 4% Paraformaldehyde and 0.2% Triton X-100 respectively for 10 min each, washed with PBS and actin was visualised by staining with Alexa Flour-488 Phalloidin (Invitrogen) 1 100 in PBS for 15 min followed by washes with PBS.

Slides were then washed three times with PBS (10-min each wash), and mounted with mounting medium (Vector Laboratories) in the presence or absence of DAPI (to label the nuclei).

Four washes (45 min each) in wash buffer were used to remove the unbound secondary antibody.