Sentence examples for min denaturation step from inspiring English sources

A 10 min denaturation step was followed by 45 thermal cycles consisting of a 10 sec denaturation step at 95°C, annealing step of 4 sec at 60°C and an extension step of 3 sec at 72°C.

The 5' and 3' RACE consisted of an initial 2 min denaturation step (94°C) followed by 35 cycles of 94°C for 15 s, 63°C for 30 s and 68°C for 70 s and then a final step at 68°C for 10 min. The 5' and 3' RACE were done using KOD plus (Toyobo, Japan).

The PCR consisted of an initial 2 min denaturation step (94°C) followed by 35 cycles of 94°C for 15 s, 50°C for 30 s and 68°C for 1.5 min and then a final step at 68°C for 10 min. The amplified DNA fragments named PsEHa were sequenced.

The PCR consisted of an initial 2 min denaturation step (94°C) followed by 35 cycles of 94°C for 30 s (denaturation), 50°C for 30 s (annealing) and 72°C for 1 min (extension) and then a final extension at 72°C for 7 min. The PCR was done using Blend Taq polymerase (Toyobo, Japan).

A 10 min denaturation step at 94°C was followed by 45 cycles of denaturation at 94°C for 30 s and annealing/extension at 67°C for 60 s.

The amplification conditions for chiA were comprised of an initial 2 min denaturation step at 94°C followed by 30 cycles of 30 s at 94°C, 30 s at 62°C and 30 s at 72°C.

The conditions for sod1, sod2, and sod3 PCRs were as follows: initial 1-min denaturation step at 94°C, another 1-min denaturation step at 94°C, 1-min annealing step at 60°C, 1-min extension step at 72°C for 30 cycles, and a final 10-min extension at 72°C.

The conditions for the β-actin PCR were as follows: initial 1-min denaturation step at 94°C, another 1-min denaturation step at 94°C, 1-min annealing step at 54°C, 1-min extension step at 72°C for 35 cycles, and a final 10-min extension at 72°C.

All PCRs began with a 5-min denaturation step at 94 95 °C, followed by 25 30 thermal cycles including a 94 °C for 20 s denaturation step, 57 °C for 20 s primer annealing and elongation step 72 °C for 1.5 min.

Conditions for β-actin PCR were as follows: an initial 1-min denaturation step at 94 °C; 1 min at 94 °C, a 1-min annealing step at 54 °C, and a 1-min extension step at 72 °C for 35 cycles; and a final 10-min extension at 72 °C.

Conditions for sod 1, sod 2 and sod 3 PCR were as follows: an initial 1-min denaturation step at 94 °C; 1 min at 94 °C, a 1-min annealing step at 60 °C, and a 1-min extension step at 72 °C for 30 cycles; and a final 10 min extension at 72 °C.