Data Summary of measurements of renal function in kidneys and single nephrons of wild type, Akita, and Akita/A1AR-/ mice Units length of fluid column, nl total fluid volume collected, min collection time, flow flow rate (nl/min), Ucpm I125 counts per minute in total fluid volume, Bkg I125 background, column H net cpm (Ucpm-Bkg), TF tubular fluid (cpm/nl).
Single experiments in Akita/A1AR-/ (DKO) mice Units length of fluid column, nl total fluid volume collected, min collection time, flow flow rate (nl/min), Ucpm I125 counts per minute in total fluid volume, Bkg I125 background, column H net cpm (Ucpm-Bkg), TF tubular fluid (cpm/nl).
Single experiments in wild type (WT) mice Units length of fluid column, nl total fluid volume collected, min collection time, flow flow rate (nl/min), Ucpm I125 counts per minute in total fluid volume, Bkg I125 background, column H net cpm (Ucpm-Bkg), TF tubular fluid (cpm/nl).
Single experiments in Akita mice Units length of fluid column, nl total fluid volume collected, min collection time, flow flow rate (nl/min), Ucpm I125 counts per minute in total fluid volume, Bkg I125 background, column H net cpm (Ucpm-Bkg), TF tubular fluid (cpm/nl).
Wilson patients showed a significant reduction in the sweat volume produced during the 30 min collection time.
Multiplication of the sweat volume produced during the 30 min collection time with the copper concentration measured in the sweat sample gives the total copper excretion.
For chromosome targeting, precellular blastoderm stage embryos (<40-min collection time) were injected with targetron Y18a or Y3776s RNPs, which contain Ll.LtrB introns targeted to insert at sites in the y gene.
Instead, we decided to use a short 1-min collection time that resulted in only a 10-fold increase in flow rate, but would require a shorter attention span on the part of students and allow sufficiently large collecting volumes for visualization.
The electrophoresis conditions for CE-FLA were 5-s injection time, 15-kV injection voltage, 15-kV electrophoresis voltage, 150-s syringe pump time, 120-s preinjection electrophoresis, and 20-min collection time for each sample, and the run was performed at 60°C.
The electrophoresis conditions for CE-SSCP were 5-s injection time, 15-kV injection voltage, 13-kV electrophoresis voltage, 30-s syringe pump time with no preinjection time, and 20-min collection time for each sample, and the run was performed at 30°C.
Sampling the 50 μL required for flow cytometry from a 1 mL vessel would require close to 5 min of collection time from effluent.
