The SEM micrographs of nano-fibrous PCL substrate prepared by electrospinning at (a) 10 min collecting time; (b) 40 min collecting time; (c) 70 min collecting time.
In unmodified substrate (10 min collecting time), growth rate is still increasing unlike other modified and unmodified nano-fibrous substrates (40 min collecting time).
After 14 days on the substrate with 70 min collecting time, the rate of cellular proliferation shows a slower process.
The substrate with 70 min collecting time illustrated highest progressive calcium deposition at all the time points.
Images from alizarin red staining show a substrate with 70 min collecting time has maximum sedimentation (Fig. 5).
As shown in Fig. 6, the substrate with 70 min collecting time resulted in highest calcification of OSTs substrates.
Data Summary of measurements of renal function in kidneys and single nephrons of wild type, Akita, and Akita/A1AR-/ mice Units length of fluid column, nl total fluid volume collected, min collection time, flow flow rate (nl/min), Ucpm I125 counts per minute in total fluid volume, Bkg I125 background, column H net cpm (Ucpm-Bkg), TF tubular fluid (cpm/nl).
Single experiments in Akita/A1AR-/ (DKO) mice Units length of fluid column, nl total fluid volume collected, min collection time, flow flow rate (nl/min), Ucpm I125 counts per minute in total fluid volume, Bkg I125 background, column H net cpm (Ucpm-Bkg), TF tubular fluid (cpm/nl).
Single experiments in Akita mice Units length of fluid column, nl total fluid volume collected, min collection time, flow flow rate (nl/min), Ucpm I125 counts per minute in total fluid volume, Bkg I125 background, column H net cpm (Ucpm-Bkg), TF tubular fluid (cpm/nl).
Single experiments in wild type (WT) mice Units length of fluid column, nl total fluid volume collected, min collection time, flow flow rate (nl/min), Ucpm I125 counts per minute in total fluid volume, Bkg I125 background, column H net cpm (Ucpm-Bkg), TF tubular fluid (cpm/nl).
PCL nano-fiber substrates were collected by 10, 40 and 70 min electrospinning prolonged collecting time causes formation of different surface texture which leads to different surface morphology, roughness and waviness.
