Sentence examples for min by measuring from inspiring English sources
The supernatant (1 ml) of grown culture was mixed with 500 µl of Salkowski reagent (2 % of 0.5 M FeCl3 in 35 % perchloric acid) and the concentration of IAA was estimated after 20 min by measuring the absorbance at 530 nm (Jeong et al. 2010a).
Membrane integrity was determined after exposure to D13-9001, Pand, and PMB for 20 min by measuring the uptake of SYTOX Green.
The reaction was performed at room temperature for 1 min by measuring the appearance of phenol at 270 nm with the use of continuously and automated recording spectrophotometer.
These mitochondria were analyzed for their membrane potential under basal conditions and in response to rotenone treatment (10 and 100 µM for 50 min) by measuring the uptake of the fluorescent carbocyanine dye JC-1.
Adhesion properties of AM-Calcein (Invitrogen -labelled PDAC cells were determInvitrogen -labelledr 90 min by measuring the fluorescence intensity.
Immediately after the addition of substrates, kinetic reads at 30 sec intervals were collected for 90 min by measuring the absorbance at 412 nm.
The contents were vortexed for 1 min and left to stand at room temperature for 20 min followed by measuring the absorbance at 510 nm.
The t 1/2 (t 1/2 = ln2/λ) of carbon-11 was estimated from these decay constants to be 20.43 ± 0.24 min (mean ± SD; n = 8), which is in excellent agreement with the accepted value of 20.38 min ascertained by measuring β+ emission [24].
The plate was shaken for 30 s every 3 min, followed by measuring the optical density at 600 nm.
The extent of HRP conjugate binding was detected by adding 200 μL of 0.04% o-phenylenediamine dihydrochloride (Sigma-Aldrich) in 0.05 M phosphate-citrate buffer for 30 min followed by measuring the optical density (OD) at 450 nm using a microplate reader (Molecular Devices).
After the reaction was stopped by adding 1000 μL DNS solution (1% 3, 5-dinitrosalicyclic acid, 20% potassium sodium tartrate, 1% NaOH, 0.2% phenol, 0.05% Na2SO3) to the mixtures, the samples were vortexed and boiled for 10 min, followed by measuring the absorbance of 200 μL sample at 540 nm using iMark Microplate Reader (Bio-Rad, Hercules, CA, USA).
