Sentence examples for min by adding from inspiring English sources

Fermentation time was varied from 250 to 500 min by adding various amounts of culture at 40°C.

In the remaining culture the degradation of Xrn1 was induced for 30 min by adding auxin to a final concentration of 0.5 mM.

The computation time was reduced to 400 min by adding a prescreening process, where virtual complexes with conflicts between main chains were dismissed from the grading process.

The desorption time could be greatly shortened down to about 2 min by adding the flow of water-vapor-enriched air at the beginning of the recovery stage which had been confirmed as a rapid and high-efficiency desorption process.

The reaction was carried out at 25°C for 5 min by adding ferricyanide solution, and stopped by adding 0.5 mL of ferric sulfate-Dupanol reagent (Wood et al. 1962).

Cell uptake was terminated at 5, 10, 15, 30, 60, 90, and 120 min by adding ice-cold Krebs buffer and rinsing the wells twice to wash away any unbound radioimmunoconjugates prior to lysing the cells with RIPA buffer.

Fig. 3b shows a series of consecutive cycles where the recovery times were reduced to less than 2 min by adding the flow of water-vapor-enriched air at the beginning recovery stage as follows.

As shown in Figure 4a, 50 μM of externally-added elemental sulfur could accelerate the reduction of Cr VI) by sulfide in the illite suspension (3.0 g/L): the time to near complete reduction of 40 μM Cr VI) was shortened from 75 min to 45 min by adding elemental sulfur.

Then, slides were incubated with a biotinylated secondary antibody for 15 min, then incubated with horseradish peroxidase conjugated with streptavidin for further 15 min, followed by adding 3-amino-9-ethylcarbasole (Dako Cytomation, USA) for 15 min.

For each sample, 400  μL of Lowry reagent was added and incubated at room temperature for 30 min, followed by adding 200  μL of Folin-Ciocalteu reagent incubated for 30 min.

The reaction was preincubated for 5 min at 30 °C prior to the addition of enzyme and quenched 5 min thereafter by adding one-fourth volume of acidic malachite green dye solution.