Sentence examples for min both at from inspiring English sources

A preincubation of 3 h and an incubation of 40 min, both at room temperature, were found to be optimal.

Sections were incubated in rabbit anti-little SAAS serum (1∶32,000 for 48 h) and biotinylated goat anti-rabbit IgG (1∶200; Vector Laboratories) for 60 min, both at 4°C with 3×10 min PBS rinses following primary and secondary antibody incubation.

Incubation was performed for 4 h, then UV irradiation at 350 nm for 20 min, both at 4 °C.

The eluted proteins were reduced by incubation with 3 mM TCEP for 20 min and then with 11 mM iodoacetamide for 15 min, both at room temperature.

Signals were recorded for 30 min, both at baseline and 30 min after intake of the glucose drink, the mixed-meal drink, or water.

Fig. 2A shows DAF-FM fluorescence from adult and old mice over 60 min, both at rest and where fibers were stimulated to contract for two 10 min periods (indicated by black bars).

Cells were first pre-extracted with 0.5% Triton X-100 in CSK buffer (10 mM PIPES, pH 7.0, 100 mM NaCl, 300 mM sucrose, 3 mM MgCl2 with freshly added protease inhibitors) for 5 10 min and then fixed in 3% formaldehyde in 1× PBS for 15 min, both steps at room temperature (RT).

All samples were incubated at 50°C for 1 hour and 70°C for 15 min; both reactions were then stored at −20°C.

At 20 min, both regions exhibited shorter T1 values than the remote myocardium (P < 0.001).

However, at 90 min, both enzyme forms followed the same trend and the same speed till they reached maximum relative conversion at 120 min.

At ~80 min, both components have higher intensities relative to normoxic levels but of relatively equivalent ratio as that of the normoxic condition.