Sentence examples for min before visualization from inspiring English sources

After incubation with the ABC-AP complex, slides were transferred to NBT buffer for 15 min before visualization with NBT.

Ovules were rinsed in PBS and also stained in 10 uM RhodFF (Invitrogen), washed with 1 ml PBS and incubated at room temperature for 30 min before visualization.

The presence of the FAD cofactor in intact protein samples was further confirmed by running the enzyme samples using SDS-PAGE and incubating the gel in 10% acetic acid for 10 min before visualization of fluorescent bands under a hand-held Mineralight® UV lamp (UVP, USA).

Membranes were allowed to dry before visualization.

BCIP/NBT (Sigma; 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium) substrate solution was added to the monolayer and incubated in dark for 25 min and washed before visualization.

The coverslips were removed from the medium,washed three times with PBS and fixed in freezer cold ethanol :acetone 1 : 1 for 15 min, dried and mounted in DAPI solution before visualization in a fluorescent light microscope.

Cells were stained with LysoTracker at 62.5 n M for 30 min at 37 °C and washed in fresh media before visualization or flow cytometry.

The TEM grids were then blotted and washed twice in distilled H2O before staining with uranyl acetate 2% for 2 min for visualization by TEM (JEOL JEM-1010).

Embryos were cleared in methyl salicylate (Sigma) before visualization.

The slide was incubated for 24 h before visualization under bright-field microscopy.

Embryos were mounted in 70% glycerol before visualization as described above for whole mount antibody staining.