Sentence examples for min before visualisation from inspiring English sources

Following washes (TBS/Tween and TBS), membranes were incubated with HRP conjugated anti-rabbit IgG (1∶2000, Santa Cruz, 1 hour) and subsequently with Luminol Reagent (Santa Cruz,1 min) before visualisation by ECL (Amersham Biosciences).

After washing with TBS-T for 2 h with a solution change every 15 min, membranes were incubated in secondary antibody solution containing 1 1750 goat anti-rabbit IgG, conjugated to HRP for 1 h, before visualisation using ECL-plus and quantification as previously described [ 56].

It represents oedema of the periaortic fat [29] and can be seen before visualisation of a retroperitoneal haematoma.

A major difference between this and earlier work is that before visualisation, this work classifies roof types of buildings as either flat or pitched.

Subsequently, the cells were washed three times with PBS before visualisation and image capture.

These cases required expert judgement of the raw images before visualisation of the area for measurement.

The membrane was washed in TBS-T before visualisation via the Odyssey Infrared Imaging System (LI-CORE Biosciences).

Thirty minutes before visualisation, propidium iodide (3 μL) and 1.5 μM Calcein-AM were added to each 2 mL well.

Ex vivo cultures were left to grow for 14 days and media were changed every 2 days before visualisation of bioluminescence using IVIS.

The gels were stained with Instant Blue (Expedeon) overnight at room temperature and then washed several times in distilled H2O before visualisation.

Before visualisation on the microscope, the media was exchanged for RPMI serum-free media (without phenol red) and 5  μ M Nile Red.