Sentence examples for min before used from inspiring English sources

The polyplex formulation was incubated at room temperature for 30 min before used.

Secondary antibody incubation was performed at RT for 45 min before using the Tyramide signal amplification kit (NEL700001KT, Perkin Elmer).

The samples were ultrasonicated for 15 min before using.

To avoid aggregation, these suspensions were ultrasonically processed for 15 min before using.

The obtained C60 dispersion was stored in a 4°C refrigerator and dispersed by ultrasound for 30 min before using.

The fresh working solution was prepared by mixing 25 mL acetate buffer, 2.5 mL TPTZ solution, and 2.5 mL FeCl3·6H2O solution and then equilibrating at 37°C for 15 min before using.

For the AC treatment, CIDR were not soaked in disinfectant but were steam sterilized at 121 °C and 724 mmHg for 20 min before use.

The test suspensions were sonicated for 20 min before use.

Silica particles were sonicated for 5 min and vortexed for 1 min before use.

The reactor, hydration flask and all tubing were autoclaved at 121 °C for 20 min before use.

Firstly, the collected BoNT-negative sera were kept in room temperature for 10 min before use.