Sentence examples for min before transferring from inspiring English sources

The mixture was stirred for 10 min before transferring to a 50-mL Teflon-lined stainless steel autoclave, which was later kept at 200 °C for 12 h.

Then, these solutions were all stirred for 30 min before transferring the mixture into a Teflon-lined stainless steel autoclave (DuPont, Wilmington, DE, USA) of 40-ml capacity and followed by heat treatments at 200°C for 9 h.

Briefly, a certain amount of treated P25, distilled water, and absolute ethanol were mixed with a mole ratio of 1 1 5, sonicated for 30 min before transferring to a 150-ml Teflon-lined autoclave (packing volume <80%), and then heated in an oven at 200°C for 24 h with no intentional control of ramping or cooling rate; thus, a homogeneous and stable TiO2 colloid was obtained.

Protein samples were separated by electrophoresis at 150 volts for 45 min before transferring to a PVDF membrane.

Gel was equilibrated in transfer buffer for 10 min before transferring to PVDF membrane (Biorad) at 30 V, 4 centigrade (C) for 18 hrs.

Electroporated cells were then incubated at room temperature for 10 min before transferring them into the 12-well plate with culture medium.

For the high magnification DIC images, 96-well µclear flat bottom plates (Greiner) were coated with 0.1 mg/ml poly-D-lysine (Sigma) for 5 min and allowed to air dry for 5 min before gently transferring 100 µl of culture to the well with a cut-off pipet tip.

For the confocal slices through rosette colonies, sterile, 8-well µ-slides (Ibidi, Germany) were coated with 0.1 mg/ml poly-D-lysine (Sigma) for 5 min and allowed to air dry for 5 min before gently transferring 250 µl of culture to the well with a cut-off pipet tip.

Samples were then completely dried with N2 and baked at 100 °C for 3 min before transferred into the MOCVD reactor (Aixtron 200) for the GaAs NWs growth.

In a typical experiment, 5 g I2 was dissolved in 100 g HPA solution (50 wt.%), and then a 100-mL graphene oxide solution was added and sonicated for 5 min before transferred into an oven and aged at 90°C for 12 h.

The gel sections that contained nanodiscs were excised and soaked overnight, then briefly boiled in 10% SDS (5 min) before transferred to PVDF membrane.