Sentence examples for min before tissue from inspiring English sources

For EdU pulse-chase experiments, 5 μL of 5 mg/mL of EdU (Life Technologies, Carlsbad, CA) in saline was injected intraperitoneally into anesthetized fish 30 min before tissue harvest.

Mice were fasted overnight (16 h) following the last rapamycin injection and injected with insulin for 15 min just before tissue harvesting.

Thus, in our experiment, the expression pattern of the accumulated IEG mRNA mirrors the neuronal activity pattern in the brain during the last 45 60 min before the tissue was fixed.

Nonsurgicated rats (n = 11) received i.p. injections of either saline (n = 5) or CCK (n = 6) (CCK-8, 8 μg/kg; Bachem), 90 min before perfusion and tissue was collected and processed as described above for IHC and FISH processing.

Jejunum pieces were rinsed, cut longitudinally and laid flat in 4% (w/v) PFA (paraformaldehyde) for 30 min before embedding in Tissue-Tek.

After the hearts were taken down, left ventricles were carefully cut off and gently shaken for 2 min before the non-digested tissue was filtered out (nylon mesh, 250 μm).

11 BBB breakdown was examined by injecting horseradish peroxidase (HRP) as a tracer intravenously 15 min before perfusion, then staining in tissue.

To correct for tissue plasma volume, a bolus dose of [125I]-albumin was injected 3 min before the experiment was terminated and tissue collected.

To test this hypothesis, lectins, i.e., proteins with a high affinity for mono- and oligosaccharides on the plasma membrane, were added to tissues 30 min before addition of chrysotile.

We transferred 4 g of powdered berry tissue to a 7-ml glass vial with an aluminum insert lid and thawed the tissue for 90 min before extraction.

Min Sample Volume: Tissue - 1 g tissue when available.