Sentence examples for min before storing from inspiring English sources

The exhausted medium was then centrifuged at ∼16 000  g for 10 min before storing at −80 °C, and those from BCG-treated PBMCs were designated as 'BCG supernatants' compared with the 'CONT supernatant', which were from untreated PBMCs.

The biopsy tissues were incubated immediately in Hanks' medium at 37°C, 5% CO2 for 30 min before storing at −80°C until assays for mast cell mediators were performed by the Immunopharmacology Group at the University of Southampton.

A final incubation at 37°C for 15 min was done before storing the DNA solution at −20°C.

To approach the potential impairment of STIM1 oligomerization by GSK-7975A, we applied 10 μM GSK-7975A to CFP- and YFP-STIM1 expressing cells 5 min before store-depletion.

After hemocytes settled in monolayers on slides, the PBS was sipped up using a filter paper, followed by fixing the slides in acetone for 15 min before being stored at −20°C until use.

The serum was heat-inactivated at 56 °C for 30 min before being stored at −80 °C.

The bacterial culture was incubated at 37°C for further 2 h, and cells were harvested and washed in sodium phosphate buffer by centrifugation (10000 × g, 10 min) before being stored as wet pellets at -20°C until use.

Virus supernatant was centrifuged at 500 × g at 4°C for 5 min before being stored at −80°C Reactivity of the new isolate with a panel of mAbs was compared with that of WNVKUN and WNVNY99 by using a fixed-cell ELISA (11 ).

Label everything before storing.

Drain the liquid before storing.

Check the lids before storing.