Sentence examples for min before storage from inspiring English sources

The Cu NW ink was vortexed at 10 Hz for 30 min before storage.

The washing solution was removed and the pellets were allowed to air-dry for 20 30 min. The dried pellets were resuspended in 60 µL of 1X SDS-PAGE loading buffer (see below) and incubated at 65°C for 10 min before storage or loading 17.5 µL from each onto SDS-PAGE minigels.

The pH was measured every minute, and averaged over 5 min before storage.

Finally, samples were boiled for 5 min before storage at -80°C until use.

These were heat-inactivated at 80°C for 5 min before storage at −80°C.

Media containing lentivirus was harvested and cleared by centrifugation at 3000 rpm for 3 min before storage at −80 °C.

Check that the jars are properly sealed before storage.

Sections were allowed to air dry at room temperature and were then fixed with 4% paraformaldehyde (Sigma, Dorset, UK) for 5 min, washed in PBS and then dehydrated in 70% ethanol and 95% ethanol for 5 min before final storage in fresh 95% ethanol.

The PCR amplification program was as follows: 5 min at 94°C followed by 30 cycles of 30 s at 94°C, 45 s at 52°C and 1.5 min at 72°C, followed by 7 min at 72°C before storage at 4°C.

The PCR amplification conditions were as follows: 4 min at 94°C, followed by 35 cycles of 25 s at 94°C, 30 s at 52°C and 2 min at 72°C, followed by the final extension step for 10 min at 72°C before storage at 4°C.

Both filtrates were mixed together and concentrated in vacuo at 55-60°C 55-60°Clume of 100 mL, and centoifuged at 12,000 g for 20 min. The supernatant was 1 g mL-1 SAA water extract, which was autoclavolume0 min at 115°C) befofe storage at -20°C.