Sentence examples for min before staining from inspiring English sources

The gating strategy for Treg and effector T cell populations is shown in Supplementary Fig. 4. For frozen sections, tissues were embedded in OCT (optimal cutting temperature compound), sectioned and post-fixed in 4% paraformaldehyde/PBS pH 7.4 for 10 min before staining.

All sections were post-fixed in 4% paraformaldehyde for 30 min before staining.

For immunofluorescence staining, cells were fixed by 3.7% formaldehyde, and then incubated with 0.3% Triton X-100 to improving the cell permeability for 10 min. Paraffin- or cryo-embedded tissues were sectioned and subjected to antigen retrieval in citrate buffer (pH 6.0, Sigma, USA) in microwave oven for 20 min before staining.

Sections were rinsed by cold PBS and fixed with 4% paraformaldehyde for 10 min before staining.

Cells were fixed in 2% PFA in KCM for 10 min before staining with 1 microgram/ml DAPI.

Frozen cryosections of skin from patients and volunteers or mice with the indicated genotype or treatment were fixed in ice-cold acetone for 10 min before staining.

After gently rinsed three times with washing buffer, the sections were incubated with the peroxidase-labeled polymer conjugated to goat anti-mouse IgG (Dako, Carpinteria, CA, USA) for 30 min before stained for 5 min with 3'3-diaminobenzidine tetrahydrochloride (DAB), counterstained by hematoxylin, dehydrated, and mounted in Diatex.

Coverslips were washed twice in distilled water over 10 mins before staining with 2% uranyl acetate for 1 hr in the dark.

After incubation with the virus, neuronal cells were washed twice in PBS (Sigma Chemicals) and fixed in 3.7 % paraformaldehyde/PBS (Sigma Chemicals) for 10 min at room temperature and suspended in cold acetone (−20 °C) for 5 min. Before staining, fixed neuronal cells were blocked with PBS containing 1 % bovine serum albumin (BSA) (Sigma Chemicals) for 40 min at room temperature.

Grids were then washed consecutively with TBG, TBS, and distilled water (5 min each) before staining with a saturated solution of uranyl acetate followed by lead citrate.

Methylmethacrylate was first dissolved in methoxyethyl acetate for 3 × 10 min and sections were partially rehydrated in 96%, 70%, and 40% ethanol for 4 min in each, before staining in Harris hematoxylin for 8 min. The sections were blued in tap water for 10 min and stained for 5 min in eosin.