Blood samples for pharmacokinetic analysis were taken 5 min after the end of the first infusion, 5 min before the second and the third dose and 5 min before the dose of the day after.
The adolescents rested for 2 min before the first measurement, and the second and third measurements were done with 1 min intervals.
For the study using second-order conditioning, other groups of animals were each injected with 3 μl of saline or saline containing 1 μM epinastine or 500 μM fluphenazine at 30 min before the first conditioning stage, before the second conditioning stage or before the final test in second-order conditioning.
Approximately 30 min before the first activity began each day, the second bathroom door was shut and the vent fan turned on.
Patients were randomly assigned to receive either 20 or 80 μg/kg rFVIIa or placebo 5 min before the first skin cut and any clamping procedures, with a second identical dose given 5 hours after first skin cut if time in surgery was anticipated to extend beyond 6 hours.
Four rats received scopolamine (0.2 mg/kg, s.c.; Sigma-Aldrich) 20 min before the first run, and five rats before the second run.
Animals were first allowed to explore the familiar or novel box for 1.5 min before the first trial of a day.
The 1 g in-flight controls were performed 5 min before the first parabola and the 1.8 g sample directly before the microgravity phase of the first parabola.
Tariquidar, 15 mg·kg-1, a dose that completely blocks P-gp function, was administered as a 15-min infusion 35 min before the second scan to all mice and as a 10-min infusion 20 min before the second scan to all rats, providing optimal inhibition conditions of tariquidar [9].
An unfamiliar stimulus mouse (a 12-week old NMRI female) was then introduced into the testing cage and left there for 3 min. At the end of the first encounter, the intruder was removed and left in a waiting cage for 30 min, before a second 3-min encounter began.
