Sentence examples for min before setting from inspiring English sources

To obtain the TbUAP1- 1 complex, the protein was incubated with 0.495 M compound at 4 °C for 30 min before setting up crystal trays.

The bovine PH-TH-kinase construct (residues 1 to 170 connected to residues 384 to 659) was thawed on ice and mixed with twofold excess IP3 (Avanti lipid) and inhibitor CGI1746 (Selleck Inc ,Houston TX), and was incubated at 4°C for 30 min before setting up trays.

To co-crystallize UNC-45 with the C-terminal Hsp70/Hsp90 peptide, UNC-45 was pre-incubated with the peptide AGGPTIEEVD/EDASRMEEVD (final concentration 500 μM) for 30 min at 19°C before setting up crystallization trials.

The protein peptide mixture was incubated at 4°C on a rocking platform for 30 min before setting-up of the crystallisation screens.

49 min: "Babel is a striker, not a left winger," begins Samuel Zakowski, before setting out on a 3,000 word defence of the player.

Always get enough sleep before setting sail.

Before setting off think about renewable energy.

Have health check-ups before setting off.

Make sure yours works before setting up.

Average values were calculated for three measurements using a 200 s integration time, and the solutions were allowed to thermally equilibrate for 10 min before each set of measurements.

Each PEEP level was maintained for 15 min before a set of measurements to allow for haemodynamic and respiratory stabilisation.