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The free nanoparticles were also suspended in aqueous buffer (50 mM sodium phosphate, pH 7.8) and in n-propanol and sonicated at 88 W power and a frequency of 40 Hz for 40 min before scanning them in DLS for a time period of 5 hours at regular intervals.
Following hybridization, the slides were washed twice in 2× SSC, 0.1% sodium dodecyl sulfate at 42°C for 10 min, followed by twice in 1× SSC at room temperature for 10 min, and finally twice in 0.2× SSC at room temperature for 5 min. The microarray slides were dried by centrifugation at 300×g for 10 min before scanning.
Patients rested for 80 min before scanning.
Slides were dried by centrifugation at 563 g for 1 min before scanning.
When CT is performed specifically to evaluate the stomach, the patient is given 750 ml of water approximately 15 min before scanning.
Following overnight hybridization, the slides were washed according to standard procedures [ 102] and dried by centrifugation at 500 g for 5 min before scanning.
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Slides were spin dried before scanning.
The streptavidin-phycoerythrin solution was added for a further 10 min and washed before scanning.
The urinary bladder was emptied by pressing the lower abdomen before induction of anesthesia 10 min before scan start, and animals were then fixed on the bed of the scanner.
Hybridized slides were washed in 1 × SSC/0.03% SDS for 6 min at 45 °C, followed by 0.2 × SSC for 5 min and 0.05 × SSC for 4 min, and then spin-dried before scanning.
The samples were exposed to an infrared red lamp for 30 min to remove the ethanol before scanning.
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