Sentence examples for min before reading from inspiring English sources

The particle/protein sensor solution was then added to each well and incubated for 45 min before reading.

Plates with CellTiterGlo were left out for 2 min before reading with the EnVision to allow for a plateau of the bioluminescent signal.

After thorough mixing, the mixture was added to three wells of the 384-well plate (25 μL/well) and incubated for 30 min before reading.

All samples were centrifuged at 2,500 rpm for 10 min before reading absorbance at 460 and 400 nm for MO and BB, respectively.

The plate was gently shaken for 10 min before reading the absorbance at 540 nm.

The solution was mixed and allowed to sit for 5 min before reading the absorbance at A520.

Nevertheless, all blank milk samples remained clearly negative with 2.25 as the lowest ratio obtained for a delay of 10 min after incubation before reading.

Plates were incubated at 30°C and shaken for 5 min before each reading.

The reactions were stopped by the addition of 4 μl of ADP-Glo™ Reagent 1 (Promega) with 40 min incubation, followed by 8 μl of kinase detection reagent for a further 60 min in the dark, before reading plates using a Pherastar plate reader (BMG Labtech) with a luminescence filter, at a read height of 14 mm and a 0.5 s integration time.

15 µL 2x Steady-Glo® reagent (Promega) was added per well and incubated 15 min in the dark before reading luminescence using an EnVision platereader.

The solution was incubated for 20 min at room temperature before reading the absorbance (A) at 517 nm against methanol as blank.