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As shown in Figure 4, Ca2+ release by uncaging of NP-EGTA-AM 5 min before one-trial conditioning also induced a specific LTM increase at 72 h.
injections, one 5 min before and one 40 min after the i.v. injection.
Cell pellets were harvested by centrifugation at 4500 g for 15 min, flash frozen in liquid nitrogen, and resuspended in a lysis buffer (20 mM Tris, pH 7.6, 0.4 M NaCl, 1% Triton X-100, 0.5 mM TCEP, 0.005 M Imidazole) and lysed by sonication (Branson Sonifier 450 at full power for 8 rounds of 8 s bursts with 1 min rests, and allowed to cool for 3 min before repeating one additional cycle).
She can't even wait 10 mins before mentioning one of our names SMH.
Patients were asked to keep the solution in their mouth for 5 min before swallowing, in one evening dose of 2.5 mg.
EEG recordings were performed for 30 min before (control) and one hour after PTZ (120 mg/kg, i.p.; Sigma, dissolved in saline) injection [ 11].
For inhibition experiments, cells were incubated for 30 min before analysis with one of the following inhibitors: 100 μM 2-APB for blocking IP3Rs, 0.5 μM TG for SERCA ER Ca2+ pump inactivation.
Two samples were taken before the film, one approximately 30 min before the film and one immediately before the film.
If a scope was used in the previous 7 days, a two-part 60-min incomplete decontamination procedure was performed: 20 min before use (including preparation, one soak, and cleaning) and 40 min after use (cleaning, three soaks with cleaning, drying, and storage).
The mixture was stirred for one min before testing.
Completion of all questionnaires took 5 15 min. One week before the training and 1 week after the last session, we applied a paper version of the Bern Questionnaire on Well-being (BQW) (Grob 1995).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com