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No differences were observed if the drug was added either 30 min or 60 min before observation.
Staining with ER-Tracker Blue-White DPX was carried out for 30 60 min before observation.
Roots were immersed into FM4 64 for 2 5 min before observation.
Aliquots (10 µL) were allowed to adsorb onto the mica and were kept at RT for 5 min before observation.
Cells were treated with the crosslinkers for 15 min before observation.
To visualize the acidification of endosomes, lysotracker (50 nM) was added at 30 min before observation.
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These were concealed in a hide set up at least 5 m from the nest a minimum of 30 min before observations started.
Males and females were allowed to acclimatize to their compartments for at least 30 min before observations started.
Behavioural observations and handling of technical equipment were carried out from behind a large, green curtain inside the experimental room, and to control for human disturbance, the observer quietly entered the room at least 10 min before each observation period.
Labeled platelets (100 × 10) were infused into the femoral vein over a period of 5 min and were allowed to circulate for 5 min before the observation period.
The penis was exposed by retracting the sheath and 5% xylocaine ointment was applied 30, 15 and 5 min before starting observations.
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CEO of Professional Science Editing for Scientists @ prosciediting.com