Sentence examples for min before mounting from inspiring English sources

After washing in running tap water for 5 min, slides were dehydrated in graded alcohols, cleared in xylene twice for 6 min before mounting with DPX mounting medium (BDH Poole, Dorset).

For nuclear counterstaining, tissue was exposed to propidium iodide (Sigma-Aldrich) (1 500 in TBS) for 45 min before mounting.

Nuclei were stained with 300 nM DAPI (4′,6-diamidino-2-phenylindole) in PBS for 15 min before mounting.

Nuclei were stained with Hoechst (1 10,000) in PBS for 5 min before mounting with 1.5 coverglass and Mowiol.

For Figure 8, embryos were incubated in SlowFade Gold Antifade Reagent with DAPI (Molecular Probes) for 30 min before mounting.

The tissue was then fixed for 30 min in 4% paraformaldehyde in mACSFHEPES and washed PBS (3 × 10 min) before mounting and imaging.

Finally, ovaries were rinsed at least 3 times for 5 min in PTw, dehydrated 5 min in methanol, and rinsed twice again 5 min in PTw, before mounting in 70% glycerol in PBS.

Samples were then washed 3 × 10 min with PBS containing 0.1% Triton X-100 (PBT) and 2 × 10 min in PBS before mounting in Vectashield (Vector Labs).

Dehydration using absolute alcohol was followed by a 5 min xylene rinse before mounting with pertex.

Cells/microvessels were washed again for 60 min with PBS before mounting on glass slides using Mowiol.

For all stainings, sections were finally washed 5x10 min with PBS before mounting on glass coverslips using fluorescence mounting medium (Dako, Glostrup, Denmark).