Patients received theophylline intravenously 30 min before infusion of contrast medium.
Cells were loaded with JC-1 (10 μg/ml) for 30 min before infusion of 5% CO2 and 95% N2 into the temperature-controlled moisture chamber of the Application Solution Multi-Dimensional Workstation for live cell imaging.
After washing, the labelled cells were resuspended in saline to the initial volume; two min before infusion, 0.1 ml of the labelled blood was mixed with an equal volume of Dex40 (15 mg/ml) and 0.1 ml of this tracer mix was infused in the animal in 1 min.
Standard deviation of beat-to-beat intervals (SDNN) was analysed at baseline and at end of study for 20 min in both the LD and UD; and in the UD, in addition, 30 min before infusion until after lunch on treatment days.
IL-13-PE was produced in Escherichia coli as described previously under clinical grade drug manufacturing by Insys Therapeutics (Chandler, AZ) 17. Blood samples from patients were collected at 30 min before infusion, and at 0, 5, 15, 30, 60, 90, 120, 180, 240 min, and 24 h after infusion completion on C1D1, C1D3, and C2D1 if treatment was continued.
Three consecutive baseline blood samples were taken, at 15, 10, and 5 min before infusion, and samples were taken at 0.45, 1, 2, 4, 8, 12, 24, 48, and 72 hours after infusion, from a peripheral, indwelling, venous catheter directly into a heparinized syringe (Becton Dickinson).
Finally the activity within all 10 min periods during the last 50 min was significantly lower compared to all 10 min periods before infusion of nitroglycerin (LSD test, P < 0.05 each; Figure 2a).
Bearing in mind the pharmacological properties of dexamethasone, the short-course i.v. premedication schedule given 30 min before paclitaxel infusion is unlikely to result in an adequate level of immunosuppression during the infusion of the drug.
The pre-OA-NAC group received IV NAC 15 min before OA infusion and the post-OA-NAC group received IV NAC 2 hours after OA infusion.
Complete blood count was repeated 24 and 72 h after course 1 and 24 h after course 4. International normalised ratio and APTT were repeated 4 h after DMXAA infusion on courses 1 and 4. Blood pressure and heart rate were monitored from 30 min before each infusion to 6 h afterwards.
Piperine and SCT-66 were applied 30 min before PTZ infusion.
