Sentence examples for min before image from inspiring English sources

At 30 min before image acquisition, cells were loaded with 10 μM DHE and incubated at 37°C.

Cells were incubated under culture conditions with fluorescent dyes for 30 min before image acquisition, using either 100 nM NAO (Sigma-Aldrich) or 10 nM tetramethylrhodamine, ethyl ester (TMRE; Life Technologies) in α-MEM, or 1 μM propidium iodide (PI; Sigma-Aldrich) and 1 100 Annexin V Alexa Fluor 488 (Life Technologies) in 0.01 M HEPES pH 7.4, 0.14 M NaCl, and 2.5 mM CaCl2.

Once an area was imaged while air-exposed, nitrogen was blown into the Petri dish through a pipet tip for 5 min before imaging the same area.

For localization studies in living cells, transfected cultures were maintained at 37°C and incubated with 9 µM Hoechst33342 (Sigma) for 15 min before acquiring images with a 405 nm diode laser and a 488 nm argon laser using a 63× HCX PL Apo Na 1.4 oil objective on a Leica SP2 confocal microscope.

Cells were preloaded with 50 μmol/l DHE or 5 μmol/l MitoSOX Red for 30 min before capturing images.

To stain the endosomes or lysosmes, Alexa Fluor 647-labelled transferrin (2.5 μM) or lysotracker green (10 μM, Invitrogen) was added 30 min before the images were taken.

Then, a new area was chosen and the Petri dish was exposed to only air for 5 min before being imaged.

For immunofluorescence, serial 10  μm frozen tissue sections were cut, and slides fixed in ice-cold acetone for 10 min before being imaged on a fluorescence microscope for Hoechst 33342 uptake.

Serial 10  μm frozen tissue sections were cut, and slides fixed in ice cold acetone for 10 min before being imaged on a fluorescence microscope for Hoechst 33342 uptake.

Mice were anesthetized by inhalation of 1.5 2% isofluorane (Baxter Healthcare) in an oxygen gas mixture 10 min before recording PET images.

Cells were then incubated with 9.4  μM Hoechst 33258 (Sigma-Aldrich, St . Louis MO) for 30 min in the dark at 37°C before image acquisition.