For MOSSA data, we extracted a 10- to 20-s 'artifact-free' epochs by visual inspection around 10 min before gas-off.
One patient (Case 4) had no acidosis because the intoxication was only 30 min before blood gas analysis in the emergency department.
For each infant, an EEG segment starting from 10 min before sevoflurane gas was turned off, and finishing when end-tidal sevoflurane concentration was 0% was identified.
The media used for anaerobic experiments were gassed (1 min) with N2 gas and degassed (4 min) for 4 cycles before autoclaving (Islam et al. [2006]).
Cross-sectional view of sample 1 that was not annealed (images 1 of (a) and (b)) and sample 2 that was annealed at 500°C for 30 min in nitrogen gas before anodization for alumina formation (annealed; images 2 of (a) and (b)).
The reaction section was purged with He gas for 30 min before heating.
The mixed solution was then purged with nitrogen gas for 30 min before 100 ppm of KPS initiator was added to the solution.
Then, an aqueous electrolyte containing 0.035 M LiTFSI was introduced into the cell and purged with N2 gas for 10 min before adding 4 g of the MMA- PEO 475 MMA- PEO 475ided by Sigmonomerich (St. Louis, MO, USA).
After preparation, the solution was well mixed and then bubbled with N2 gas for 15 min, before being transferred to a N2-flushed sterile serum vial through a 0.2 µm pore size sterile filter using a syringe and needle.
Two grams of yeast extract powder was added, and the mixture then bubbled with N2 gas for 15 min, before being transferred to a N2-flushed sterile serum vial through a 0.2-µm pore size sterile filter using a syringe and needle.
Samples were purged under a gentle stream of CO gas for 5 min before the addition of a 5-fold excess (5 mM) of anaerobic buffered ascorbate and then incubated under an atmosphere of pure CO for an additional 5 min. After reaction with CO, protein solutions were loaded into the IR cell (50 μm path length) at a final concentration of 1 mM in copper.
