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After 30 s, the colored powder was collected by filtration and rinsed with n-butanol then dried at 80 °C for 5 min before further characterization.

After the injection, the animals were kept in the lateral position with the injected ear oriented upward for 15 min before further measurements.

The stabilized nanofiber was carbonized at 600 °C for 2 h under a nitrogen atmosphere using a tube furnace and then crushed by mortar and pestle for 5 min before further use in this study.

Following the overnight incubation, the cells were washed with Krebs-Ringer solution and incubated with 0.1 µM N6-phenylisopropyl adenosine and 2.5 µg/mL adenosine deaminase for 10 min before further treatment.

Urine samples were normalized to their respective creatinine relative peak area of [M+H]+ = 114.0667 m/z with retention time of 0.32 min before further analysis of the data.

Supernatant was incubated at 75°C for 25 min before further centrifugation.

After exercise, participants remained at rest for 60 min before a further blood sample was collected.

These conditions were maintained for 2 min before a further linear increase of B to 90%% within 6 min and isocratic elution for 2 min.

Sections were then stained with periodic acid (0.5%, 5 min), rinsed with water and Schiff's reagent added (10 min) before a further rinse under running tap water and staining with Mayer's haematoxylin (20 s).

Glass substrates for asymmetry experiments were silanized by acid washing in 1.0 M HCl and treating with 5% dimethylsilane in xylene for 5 min before drying, further washing and sterilizing at 200°C.

In some experiments, tafamidis (100× stock in DMSO) was added to healthy donor plasma ex vivo and the sample was incubated at room temperature for 30 min before being further processed.