Sentence examples for min before fixing from inspiring English sources

The samples were cytospun onto slides at 500 r.p.m. for 5 min before fixing in 1% formaldehyde in PBS-T.

In all experiments, lymphocytes were incubated on the sections for 30 min before fixing in acetone, washing and immunohistochemical staining to detect adherent cells.

Mitotracker red CMXRos (25 nM; Life Technologies, Carlsbad, CA, USA) was added to the culture medium 30 min before fixing in 4% paraformaldehyde.

To further explore the distribution of such endosomes, COS-7 cells were loaded with fluorescent EGF for 30 min, before fixing and co-staining with the anti-PtdIns4 P antibody.

The cells were incubated in these conditions at 37°C in 5% CO2 in air for up to 180 min before fixing and staining with 5  μ M Nile Red for 5 min.

FM4-64 staining of PM was performed by incubating fat body in ice-cold PBS containing 5 mg/ml fixable FM4-64 (LiforTechnologies) for 1 min before fixing for 20 min in ice-cold PBS containing 4% PFA.

Then, 100 µM 5-BP was added to wells and co-incubated with the inhibitors for 30 mins before fixing and staining as described above.

Some neuronal cultures were incubated with 15 μM propidium iodide (Invitrogen) for 10 mins before fixing and with 0.5 μM DAPI (4′, 6-diamidino-2-phenylindole) (Sigma) for 5 mins after fixing during washing with PBS.

Cells were treated with 10  μM BrdU for 20 min minutes before fixing with 4% paraformaldehyde.

After 72 hr at 37°C, cells were allowed to bind anti-CD8 antibody at room temperature for 15 min, washed, and then chased for 30 min at 37°C before fixing and immunolabeling.

As before, fix (varepsilon>0).