Sentence examples for min before fixation from inspiring English sources

For hypotonic treatment, cells were incubated in 60 mM KCl at room temperature for 30 min before fixation.

The posterior region of embryos was bisected and one half was fixed immediately and the other half was cultured for 60 min or 120 min before fixation.

When required, cells were pre-extracted with 0.5% Triton X-100 in CSK buffer (10 mM Pipes pH 7.0, 100 mM NaCl, 3 mM MgCl2 and 300 mM sucrose) for 5 min before fixation.

For Brefeldin A (BFA) experiments, IA2.2 cells were treated with 20 µg/ml BFA for 60 min and pulsed with TMR-Dex during the last 10 min before fixation and imaging.

We did not observe any pyknotic nucleus indicating that ET (10−7 M for up to 20 min before fixation) does not induce cell death in the acute cerebellar slices at least within the duration of the experiments.

Whole brain acute sagittal slices taken from adult (P25 P30) mice were incubated in the presence of ET (10−8 and 10−7 M, at room temperature) for 5 min before fixation and then immuno-stained for ET using immunoaffinity purified primary antibodies specific for ET (see single 36 kDa ET band in Fig. 1A).

When required, 10 µM BrdU (Sigma) was added to cultures for 5 min immediately before fixation.

At indicated time points, the larval tissue was dissected from larvae in Schneider's cell culture medium (Life Technologies, Paisley, UK) within a 30 min interval before fixation.

To relate the intracellular tTG distribution to membrane compartments, the intracellular membranes were labeled for 10 min with 10 µg/ml styryl dye FM4-64FX 30 min before cell fixation and labeling with anti-tTG antibody.

Synchronized mitotic cells were cultured and allowed to progress into G1 phase for 30 min before chemical fixation as indicated.

FISH analyses were carried out primarily as described [42] except that cells were fixed in 4% paraformaldehyde (PFA) for 30 min and were permeabilized with 0.2%Triton X-100 (in PBS/DEPC) for 5 min before post-fixation in 2% PFA.