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In the final chamber, containing recording artificial CSF (as for dissection artificial CSF but with 2 mM Ca2+ and 1 mM Mg2+), slices were allowed to return to room temperature and recover for at least 40 min before experimentation.
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15 min before the experiment.
The broilers were allowed to acclimate to their experimental environment for 7 days before experimentation began.
Before experimentation, the mass flux and nozzle to plate distance were optimised.
The mice were quarantined for 1 week (12-h light/dark cycle, 22 ± 2 °C, 60 65% relative humidity) before experimentation.
Transit-time flow probes were implanted on the pulmonary and left renal arteries 2 weeks before experimentation.
Animals arrived in the vivarium 24 h before experimentation to allow them to acclimate to the environment.
Subjects gave written informed consent before experimentation.
overnight at room temperature before experimentation.
The animals were restrained from drinking the day before experimentation.
Cells were allowed to recover for 24 48 hours before experimentation.
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