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The grids were allowed to dry for an additional 10 min before examination under the electron microscope.
After fasting for at least 6 h, the patient is instructed to drink 500 to 750 ml of tap water as negative oral contrast during 15 20 min before examination and is then placed on the scanner table in either supine or prone (when antropyloric abnormalities are suspected) position.
The grids were then washed in distilled water and stained with 2% ethanolic uranyl acetate for 10 min and with lead citrate for 2 min before examination in a FEI Tecnai 12 TEM [63].
One of the examiners placed a bacitracin tablet in the selective culture broth about 15 min before examination.
Two drops of mydriatic eye solution (Mydriacyl®, Tropicamide, 1%) were applied to each eye 15 min before examination of the fundus.
Oral administration of water or contrast (2%) was given 20 min before examination and contrast enema (2%) was given immediately before examination.
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These measures were completed approximately 30 min before each examination.
The carbon membrane was dried in infrared light for 20 min before TEM examination.
The subjects were requested to refrain from eating and drinking for at least 12 h and from smoking for at least 30 min before the examination.
Each patient was instructed to fast for 6 h before undergoing PET and was intravenously injected with 370 MBq (10 mCi) of 18F-FDG 60 min before the examination.
NAC (China Chemical & Pharmaceutical, Taipei, Taiwan) was intravenously administered at a single dose of 3 mg/mouse, 30 min before microscopic examination.
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