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Cannulas were left in place for 5 min and mice remained facing the CD1 for another 11 min (i.e., total time of visual and olfactory contacts of 20 min) before entering the second brief social defeat session.
Three drops of tropicamidum 0.5% (Tropicol®) were administered in the eyes 20 min before entering the scanner to inhibit pupillary constriction.
All of these drugs were administered 60 min before entering the operating room.
All patients were premedicated with intramuscular morphine (0.1 mg/kg) and scopolamine (0.005 mg/kg) 60 min before entering the operating room.
The exhaust was further diluted by high efficiency particulate air filtered air (FA) and then aged for 4 min before entering the 4 ft × 6 ft × 7 ft exposure booth.
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By plotting R over time in 15 cells that spend at least 7 min in metaphase before entering anaphase, we could directly observe how cells centered the plate position over time, while reducing the variability in metaphase plate position.
Additionally, BPo and HRo (oscillometric BP and HR) (Omron M5-I; Omron Healthcare) were measured at t−30 min (supine and upright) before entering the elevator, on the platform when harnessed at t−1 min (upright), following the jump at t+10 min (upright) and t+120 min (supine and upright).
During the spray all meals and liquid was taken out and the owner was advised to keep the door open after collection for at least 30 min before re-entering the house.
All participants were injected with 370 MBq C-PiB at rest before entering the scanner 30 min later.
DLCO was measured for each subject before entering the exposure chamber and 20 min after leaving the exposure chamber.
Before entering the laboratory, subjects performed a 5-min walk warm-up on the outdoor sports court followed by instruction and training on proper stretching exercises.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com