Sentence examples for min before cold from inspiring English sources
To assess baseline mediator levels, dialysate was collected from both probes for 30 min before cold provocation (4°C for 5 min) of the skin above one fibre.
Animals were kept on ice for 10 min before fixation with cold 2.5% glutaraldehyde, 3% paraformaldehyde with 5% sucrose in 0.1 M sodium cacodylate buffer (pH 7.4) overnight, then post-fixed in 1% OsO4 in veronal-acetate buffer.
Stromal-cell-derived factor-1 α was added to cells at a final concentration of 10 n for 0 20 min before dilution in cold PBS containing 1 m sodium vanadate and cells were harvested.
Sections were denatured in 70% formamide (in 2 × SSC, pH 7.0) at 75°C for 2 min before quenching in ice-cold ethanol.
Samples were incubated for 1 h at RT to prevent urea derived carbamylation of lysine side chains, then were alkylated by addition of iodoacetamide (375 mM) in TEAB for 30 min before precipitation with 1 ml cold (−20°C) acetone and kept at −20°C overnight.
The tubes were then heated in a boiling water bath (80°C 100°C) for 15 min before being transferred to a cold water bath.
After different drug treatment, the cells were stimulated with 10 ng/ml EGF for 15 min before harvesting, washed twice with cold PBS and lysed with RIPA buffer that containing protease and phosphotase inhibitors cocktail (Roche, UK).
For staining of cryo-sections, sections were fixed first with cold acetone (10 min), before probing with anti-Irga6 antiserum 165/3 or anti-TCR Vβ8.1/8.2 mAb (BD Pharmingen, CA, USA).
After 20 days, when the growing tumour had reached about 2 cm in diameter, five mice were intravenously administered 50 μg of cold OT 30 min before the radiotracer and the other five were not.
All hearts were subjected to 30 min of cold ischemia (+4 °C) and 6 h of RP before explantation.
FM4-64 staining of PM was performed by incubating fat body in ice-cold PBS containing 5 mg/ml fixable FM4-64 (LiforTechnologies) for 1 min before fixing for 20 min in ice-cold PBS containing 4% PFA.
