Sentence examples for min before chilling from inspiring English sources

After two washes in PBS the cells were resuspended in 2 ml of lysis buffer (PBS, 1 mg/ml DNase I, 100 µM benzamidine, 100 µM PMSF, 1 mg/ml RNase, 2 mg/ml lysostaphin) and incubated at 37°C for 20 min before chilling on ice.

Leave to cool before chilling.

In brief, random hexamer primers and deoxyribonucleoside5'-triphosphates (dNTPs) were added to 5 µg total RNA and the mixture was incubated at 65°C for 5 min before brief chilling on ice.

10 × 10 cells in 15 ml tubes were re-suspended in 600 µl TES (10 mM Tris pH 7.5, 10 mM EDTA, 0.5% SDS) and 600 µl phenol pH 7. The mixture was incubated at 65°C for 20 min with 30 s vortexing every 5 min, before briefly chilling on ice.

Formamide loading dye (100% formamide with 0.25% bromophenol blue, 0.25% xylene cyanol) was added (5 µl) and the sample heated at 95°C for 2 min before being chilled on ice.

It takes about 20 minutes before chilling.

Then strain again into a pitcher before chilling.

Before chilling, all the flower buds were dated and monitored up to 2 days before anthesis.

Add any sugar before chilling your coffee.

Cool completely before chilling in the refrigerator overnight.

Allow to cool completely before chilling it in the refrigerator.