Sentence examples for min before cells from inspiring English sources

Propidium iodide (PI) (1.4 μg/ml) was added 5 min before cells were analyzed using an LSRII flow cytometer (BD Biosciences, San Jose, CA, USA).

Preincubation with JNK inhibitor, SP600125 (40  μM), or/and ERK inhibitor, PD98059 (60  μM), was carried out for 30 min before cells were treated with 50  μM FK506.

Aliquots of cells were incubated with fluo-4 AM (10 μM) for 20 min, and this mixture was allowed to de-esterify for at least 30 min before cells were used.

SPBs were allowed to separate for 1 hr 30 min before cells were treated with nocodazole for an additional 30 min. After 2 hr total, nocodazole was washed out and frames were grabbed at approximately 94 s intervals for a total of 21 frames.

SKBR3 cells were preincubated alternately at pHe 7.4 or pHe 6.8 for 30 min, before cells were exposed to medium containing prolactin or vehicle for 15 min at either the same pHe or the alternate pHe, and prolactin-induced Stat5, Jak2 and Erk signals were examined.

To determine the minimum amount of time required for cells to be subjected to a metabolic precursor to produce modified cell-surface glycans that could be detected by click chemistry, we performed a pulse-chase experiment, in which Jurkat cells were treated with Ac4ManNAl (50 μM) for 1, 5, or 10 min before cells were washed and transferred to the culture medium without the unnatural sugar.

In order to obtain standard spherical particles and decrease defects on the surface, the AgNP-decorated SiNW array was annealed in N2 at 200°C for 90 min before cell fabrication.

We serum starved the cells for 24 h and added IGF1 either during the whole starvation period or during the last 10 min before cell lysis (Figure 5C).

To relate the intracellular tTG distribution to membrane compartments, the intracellular membranes were labeled for 10 min with 10 µg/ml styryl dye FM4-64FX 30 min before cell fixation and labeling with anti-tTG antibody.

Approximately 90 min before cell harvest, the lymphocyte culture was supplemented with 500 ng/ml colcemid.

Samples were incubated in the dark for 30 min before cell cycle analysis.