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Solutions were stirred for 30 min before being allowed to age for an additional 23.5 h.
Animals were placed in the maze for 5 min before being returned to the home cage.
The needle was withdrawn of one coordinate and left for further 2 min before being totally removed.
H4bptc (33 mg, 0.1 mmol) was then added to the solution which was sonicated for 5 min before being moved to a preheated oven at 120 °C.
The M-FISH probe (10 μl for each 22 × 22-mm hybridization area) was denatured at 65 °C for 10 min before being applied onto the denatured slides.
Animals were injected intraperitoneally with saline or 1 mg/kg CNO 35 min before being placed in the open-field arena for 5 min.
H4abtc (35.8 mg, 0.1 mmol) was then added to the solution which was sonicated for 5 min before being moved to a preheated oven at 120 °C.
After each injection, the needle was left in situ for 5 min before being retracted, to allow complete diffusion of the spheres.
The deposited gel bed was solidified at 37 °C for 30 min before being incubated in warm DMEM/F12 at 37 °C for 24 h before use.
The samples were subsequently exposed to Pearl's solution for 30 min before being washed with water.
After injection, the needle was left in place for 1 min before being slowly withdrawn.
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CEO of Professional Science Editing for Scientists @ prosciediting.com