Sentence examples for min before assessment from inspiring English sources

In order to assess the lethality of the Sox2 siRNA phenotype, day 5 siRNAsiRNA embryos were incubated in Trypan Blue (Sigma) solution, diluted 1∶10 in KSOM embryo culture medium at room temperature for 10 min before assessment by bright-field microscopy.

Subjects had to rest supine for at least 5 min before assessment.

Incubation of isolated GR (2.5 μM protein) with 0 200 μM HOSCN was carried out for either 15 or 120 min, before assessment of enzyme activity.

In all further experiments (Fig. 1), K201 was added to the solution at least 60 min before assessment of muscles strip function.

Inclusion criteria were as follows: age 4 to 12 years; hospitalized in surgical or medical wards; presenting a pain condition (post-operative or disease-related pain); no analgesics administered within 30 min before assessment (except constant infusion); willing to participate.

In the first experiment, saline or 100 mg/kg glucose was administered 10 min before cognition assessment.

All subjects rested for at least 15 min before cardiovascular assessment as described below.

Olanzapine (0.5, 1 and 2 mg/kg) and saline were injected to the rats intraperitoneally 30 min before ethanol withdrawal assessment.

For wounds that continued to bleed, fresh pieces of gauze were applied for 2 min before removal and assessment.

For condensed chromatin, 1  μg/ml of Hoescht 33342 was added to media 20 min before the live assessment, cells were washed twice in PBS pH 7.5 and fresh media was re-applied.

Isolated PTP1B (0.5 μM) was incubated with 0 25 μM HOSCN (generated enzymatically using a LPO/H2O2/SCN− system; see the Experimental section) for 5 min at 22 °C (pH 7.4), before assessment of the remaining enzyme activity using the conversion of p-nitrophenyl phosphate into p-nitrophenolate anion, with the latter quantified spectrophotometrically at 405 nm.