Sentence examples for min before adding from inspiring English sources

For blocking experiments, cells were pre-incubated with 1 μM non-labelled ZEGFR:2377-ST for 5 min before adding 0.1 μM ZEGFR:2377-ST-[DyLight488].

We added one of the C60 derivatives or NAC at various concentrations 30 min before adding the IL-4 and anti-CD40.

The reaction was incubated for 5 min at 95 °C and cooled to ambient temperature for 2 min before adding 50 mM DTPA [17].

Samples were then shaken vigorously and mixed on a rotary extractor (Caframo REAX) for 15 min. After extraction, tubes were placed in a sonication bath for 15 min before adding 4 mL of Milli-Q water to each tube.

The dye solution was then vortexed for 15 min before adding 100 µL culture medium.

To inhibit RNA pol II, α-amanitin (200 µg/ml; Sigma-Aldrich) was added to the suspension of nuclei 10 min before adding the transcription mix.

Medium supernatants were removed from MSC cultures prior to cell harvest and sedimented at 400 g for 10 min before adding to MLR cultures at the indicated dilutions.

In control experiments, the proteasome was inhibited by addition of 50 µM MG 132 to cell lysates 10 min before adding the fluorogenic substrate.

Where indicated, dec-RVKR-cmk (25 µM) and aprotinin (100 µM) were added to the cells 20 min before adding bPA83/bPA63.

Briefly, slices were incubated in H2O2 3% for 20 min before adding the blocking mix.

TMB substrate was allowed to incubate for 5 min before adding the stop solution.