Sentence examples for min before activation from inspiring English sources
After the addition of ground extract, vials were incubated for 5 min before activation with 0.5 mM RuDP (ribulose 1,5-diphosphate).
A half-volume of the PRP obtained was frozen at −30°C for 2 h and then thawed in a dry thermostat at 37°C for 30 min just before activation (frozen PRP).
The same PCR conditions were used except an addition of initial incubation at 37°C for 30 min before the activation of HotStarTaq polymerase.
Freshly isolated human islets plated on dishes coated with extracellular matrix were loaded with 10 μmol/l YO-PRO-1 10 min before receptor activation by addition of 100 μmol/l benzoyl ATP (BzATP).
After pretreated with PRT062607 and Piceatannol for 30 min before A. fumigatus hyphae stimulation, activation of Syk was inhibited by 1 μM PRT062607 (p < 0.05), 2 μM PRT062607 (p < 0.01), 5 μM Piceatannol (p < 0.05), 10 μM Piceatannol (p < 0.01) (Fig. 3a) compared with untreated cells.
Comparison of mRNA levels of P. falciparum gametocytes before and 30 min following activation using suppression subtractive hybridization (SSH) identified 126 genes, which changed in expression during gametogenesis.
The presence of 3 nmol S1RA, administered 30 min before morphine treatment, nearly abolished CaMKII activation, reduced NR1/NR2 phosphorylation, and preserved the MOR-NR1 inhibitory association during the intervals that corresponded to the morphine analgesic time-course and beyond (Fig. 5C and Supplementary Fig. S4).
Total STAT3 and STAT3 pY705 levels were measured in JJN3 cells pre-treated with 1 or 2 μ niclosamide for 2 h before activation of the STAT3 signalling pathway with interleukin 6 for 15 min.
The optimal activated carbon is obtained when using 68 min as activation time and 1095 K as activation temperature.
Patients were prepared with a fasting period of 6 h and 10 mg Valium per os 10 min before FDG administration to avoid brown fat activation.
Open image in new window Fig. 4 Smart LCM before activation (left) and after activation (right).
