Sentence examples for min at varying from inspiring English sources

After the enzymes were incubated in the buffer solutions (pH 8.5) for 60 min at varying temperatures, the remaining activities were measured at 37°C.

The optimum temperature was determined by incubation of the recombinant enzyme in the presence of 50 mM sodium phosphate buffer pH 6.0 and 2 mM 4-nitrophenyl β-xylopyranoside (PNPX) for 15 min at varying temperatures between 20°C and 90°C.

Each session lasted 50 ± 2 min at varying intensities between 40 and 80% of pre-bed rest maximum oxygen uptake.

The thermal stability of BmooMP α-II was tested by preincubating the enzyme (10  μg) in saline, pH 8.0, for 15 min at varying temperatures (30 90°C).

Both the free and xerogel entrapped LiP were incubated with citrate phosphate buffer of pH 6.0 and 5.0, respectively, for 15 min at varying temperatures 20-80°C 20-80°Ce running the enzyme assay.

To determine the optimum temperature and optimum pH for enzyme activity, several assays are performed at pH 8.5 for 30 min at varying temperatures (4, 23, 30, 37, 45, 50, 55, 60, 70, 80 and 90°C), and at 37°C for 30 min with buffer of various of pH (5, 6, 7, 7.5, 8, 8.5, 9, 10, 11), respectively.

PCR conditions for the latter comprised a pre-denaturation step at 94°C for 2 min, followed by 40 cycles each with denaturation at 94°C for 1 min, annealing at varying temperatures (Additional file 9) for 1 min, and extension at 72°C for 2 min. At the end, a final extension step at 72°C for 5 min was added.

A known mass of adsorbent ranging from 20 to 100 mg was added to the different 250 mL conical flasks that contained 20 mL solution of each adsorbate and the mixture was agitated at 160 rpm on the mechanical shaker for varying times of 5 360 min and at varying temperatures of 30 60 °C.

Additional file 5: Ex vivo biodistribution data for [18F]BF4 − in BALB/c mice 45 min post-injection at varying doses of BF4 −. (PDF 18.1 KB).

Fig. 2 Side and anterior PET/CT maximum intensity projections of a normal BALB/c mouse 25 30 min post-injection of [18F]BF4 − (2.5 MBq, SA = 5 GBq/μmol) showing uptake in the thyroid, stomach, salivary gland and olfactory mucosa Fig. 3 Ex vivo biodistribution data for [18F]BF4 − in BALB/c mice 45 min post-injection at varying doses of BF4 − (n = 3 for each dose) showing data for all tissues.

The kinetics and dose-response relationship of this interaction were determined by measuring residual GSH and residual BCNU-cytotoxicity in aGSH/BCNU mixture over a 45-min period and at varying BCNU concentrations.