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The stability and metallurgical failure mechanism for TaRhx diffusion barriers in contact with Cu and/or Si were investigated by resistivity measurements, XRD analysis and detailed electron microscopy on samples annealed in 5%H2/95%% N2 gas for 30 min at various temperatures.
The reaction was carried out at 35°C for 10 min at various pH values (open symbols).
The enzymes were incubated for 60 min at various temperatures and the residual activities were assayed under standard conditions.
Figure 1 SEM images of Au-catalyzed SiGe NW grown for 40 min at various temperatures with R = 0.15.
The nanopowders were sintered using a hot pressing facility with a direct current under a pressure of 30 MPa and held for 2 min at various temperatures.
ZnO NRs grown on Si substrates were annealed in nitrogen gas with 1 × 102 Torr for 20 min at various temperatures (200°C to 800°C) in a rapid thermal annealing furnace.
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Therefore, the minimum required generator temperature (Tgen-min) at various nozzle exit Mach numbers and the largest possible nozzle exit diameter for one particular ejector are provided for this present work.
The PCR reactions were as follows; initial denaturation at 95°C for 2 min, 30 cycles of; denaturation at 95°C for 1 min; annealing at various temperatures for 1 min; elongation at 72°C for 2 min, and a final elongation at 72°C for 5 min. Reactions were performed using a T Gradient Thermocycler (Biometra GmbH, Goettinger, Germany).
The tumours were then heated at 40 degrees C for 60 min. At various time points after heating, tumour-bearing mice were irradiated while alive or after being killed.
Briefly, developed cells were resuspended at 3 × 107 cells/mL with 3 mM caffeine in PM buffer (10 mM phosphate buffer, 2 mM MgSO4) and shaken at 200 rpm for 15 30 min. At various times after 1- μM cAMP addition, 100- μL aliquots were collected, fixed and stained with TRITC-phalloidin.
When cleaved complexes, formed by incubation of drug, gyrase, and plasmid DNA, were subjected to 5 min incubations at various temperatures, the production of linear DNA showed a distinct decrease over the same temperature range at which supercoiled DNA increased (Supporting Information Figure S5; the fraction of nicked DNA grew slightly as temperature increased).
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