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The microplate was incubated for 30 min at room temperature.
Cells were fixed with 1% formaldehyde for 20 min at room temperature.
Next, the slides were counterstained with Mayer's haematoxylin for 5 min at room temperature.
Samples were incubated with the probes in MiR05 for 30 min at room temperature.
The lungs were incubated for 40 min at room temperature and minced thoroughly.
Blood was collected by cardiac puncture and allowed to clot for 30 min at room temperature.
Cells plated on coverslips were fixed with 4% paraformaldehyde (20 min at room temperature (RT)).
For ChIP-seq, sorted cells were crosslinked with 1% formaldehyde for 10 min at room temperature.
Briefly, cells were harvested and crosslinked with 1% formaldehyde for 10 min at room temperature.
After 3 h, cells were fixed with 4% paraformaldehyde for 30 min at room temperature.
All cells were fixed in 4% paraformaldehyde for 10 min at room temperature.
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