Ten milliliters of hexane was added to all tubes, followed by ultra-sonication for 60 min at room temperature, and then vortex for 5 min at maximum speed.
The device measures 5.1 cm×8.1 cm×12.4 cm, weighs 235 g, and is powered from rechargeable lithium ion batteries with a lifespan of 45 min at maximum transmission power.
After cooling down on ice for 5 min, the medium was boiled at 98°C for 10 min and centrifuged during 10 min at maximum speed to inactivate the enzyme.
In brief, SH-SY5Y cells were homogenized on ice, in 2 ml 0.06 M Tris HCl-buffer pH 7.0 (assay buffer Tris HCl-bufferentire pHocedure) for approximately 1 min at maximum speed using a Polytron® homogenizer (Kinematica, AG).
The SBA-Pr-NH2 (0.01 g) was activated in vacuum at 100 °C and then after cooling to room temperature, 2-amino-3-hydroxypyridine (3 mmol, 0.33 g) and appropriate benzoyl chloride (3 mmol) were mixed in a glass tube and irradiated in the microwave oven for about 5 min at maximum microwave power level (700 W).
The 4 mL concentrated cell solution was centrifuged for 15 min at maximum speed.
It was then heated for another 5 min at maximum pressure.
The water was poured out and the cell pellet was transferred with 1 mL of sterile seawater to a 2 mL tube and centrifuged for another 10 min at maximum speed.
In brief, the sections were deparaffinized in xylene, hydrated in gradient alcohol, and pre-treated in a microwave oven for 15 min at maximum power in citrate buffer (0.01 M, pH = 6.0, 0.5% Tween-20) for antigen retrieval.
Bacterial cultures were grown to an optical density of 0.5 (600 nm) in LB broth, and 200 µl from each culture were mixed and centrifuged for 5 min at maximum speed.
Buffer ASL (1.6 ml) from QIAamp DNA Stool Mini Kit (Qiagen, Hilden, Germany) was added to each sample, after which the samples were homogenized in a MiniBeadbeater-8 (BioSpec Products, OK, USA) for 2 min at maximum speed.
