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Heating whey protein dispersions (90°C for 15 min) at low ionic strength and pH values far from isoelectric point (pH>6.5) induced the formation of soluble polymers.

No further changes were apparent in skeletons maintained in this buffer for up to 40 min at low temperatures (T less than 10 degrees C), but skeletons shrank when the ionic strength of the buffer was increased.

Beads were centrifuged for 1 min at low speed, washed three times (20 mM Tris, 300 mM NaCl, 0.1% Tween-20, pH 8.0), and incubated with 30 μL Laemmli buffer.

The mixture was chopped for 20 min at low temperature to obtain a homogeneous sol.

Then water and cement were mixed into blender for 1 min at low rpm.

The (27.12 MHz) plasma treatment time was from 5 to 100 min at low pressure, ∼2 Pa.

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IrC1 specifically stained the nuclei of dead cells over living cells rapidly (<10 min) and at low concentrations (10 μM), as observed using confocal luminescence microscopy.

Figure 5 Raman spectra of 10−6M CuTMPy measured on Ag/Si nanoisland films, annealing time 15 min. At low signal intensities from the analyte molecule, like in Fig. 5, we could observe two additional features centred near 1,350 and 1,600 cm−1, correspondingly.

Optimization of printing parameters allows for a geometrically accurate 3D patient-specific AAA model to be produced rapidly (in 25 h and 3 min) and at low cost (£4.50 per model) using a desktop FDM machine.

The cell suspension was centrifuged 2 min. at low speed in a clinical centrifuge and the cell pellet was returned to fresh culture medium.

Area under the curve (AUC) for glucose production was the primary outcome, estimated over 60 min. At low insulin levels, EGP rose proportionately with glucagon dose, from 5 ± 68 to 112 ± 152 mg/kg (P = 0.038 linear trend), whereas at high levels, there was no increase in glucose output (19 ± 53 to 26 ± 38 mg/kg, P = NS).

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